HPLC Resolution Calculator
HPLC resolution calculator
Calculate chromatographic resolution between two peaks.
InputsChemistry4 fieldsLive
Result
Rs = 2.66666667
Uses Rs = 2(tR2 - tR1)/(w1 + w2).
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Chem signal
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Formula
Rs = 2(tR2 - tR1)/(w1 + w2) Symbol legend
| Symbol | Meaning | Unit | Copy |
|---|---|---|---|
R_s | Chromatographic resolution | - | |
t_{R1}, t_{R2} | Retention times for peaks 1 and 2 | time | |
w_1, w_2 | Baseline peak widths | time |
- Enter retention times for two adjacent peaks with peak 2 eluting later.
- Enter corresponding baseline widths.
- Apply equation to compute resolution Rs.
Example
Worked example: tR1=2.5, tR2=3.1, w1=0.20, w2=0.25
- 1 Numerator = 2(3.1 - 2.5) = 1.2
- 2 Denominator = 0.20 + 0.25 = 0.45
- 3 Rs = 1.2/0.45 = 2.667
Resolution is approximately 2.67.
How
- Enter tR1, tR2, and baseline widths w1, w2.
- Confirm tR2 is larger than tR1.
- Read computed Rs value.
Avoid
- Entering peak order backward so tR2 <= tR1.
- Mixing width definitions (baseline vs half-height).
- Using inconsistent time units between tR and w values.
Ref only. Verify values. Follow lab safety.
FAQ
What Rs is often considered baseline separation?
A common practical target is Rs around 1.5 or greater.
Can I use seconds instead of minutes?
Yes, any consistent time unit is acceptable.
Does this include asymmetry corrections?
No, this is the standard basic resolution equation.
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