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Calibration Curve Calculator

Calibration curve calculator

Estimate concentration from linear response y = mx + b.

InputsChemistry3 fieldsLive

Result

1.4 concentration units

Uses x = (y - b)/m.

Live update

Chem signal

Advanced options
Formula
y = mx + b
so x = (y - b)/m

Symbol legend

Symbol Meaning Unit Copy
y Measured instrument signal instrument units
m Calibration slope signal/concentration
b Calibration intercept signal units
x Calculated concentration concentration units
  • Use signal value y from sample measurement.
  • Use slope m and intercept b from calibration line.
  • Rearrange linear equation to solve x concentration.
Example

Worked example: y = 0.75, m = 0.50, b = 0.05

  1. 1 x = (y - b)/m
  2. 2 x = (0.75 - 0.05)/0.50
  3. 3 x = 1.40

Calculated concentration is 1.40 (in calibration units).

How
  1. Enter measured signal y.
  2. Enter calibration slope m and intercept b.
  3. Read estimated concentration x.
Avoid
  • Entering slope with wrong sign.
  • Forgetting to subtract intercept before division.
  • Using a non-linear method with this linear calculator.

Ref only. Verify values. Follow lab safety.

FAQ
What if slope is zero?

A zero slope means no calibration sensitivity; concentration cannot be solved by this form.

Can I use this for negative intercept values?

Yes, as long as slope is non-zero and values are meaningful for your method.

Does this tool fit a calibration line?

No, it assumes slope and intercept are already known.

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